Introduction
Innovative Research’s human cystatin C purified 10X C-terminal histidine tag has been recombinantly produced in insect cell culture and purified. This is a frozen liquid buffered in 0.01 M sodium phosphate; 0.3M NaCl; pH 7.0, with a concentration of 1.0 mg/mL and greater than >95% purity as determined by SDS-PAGE analysis. This product can be used as a positive control for ELISA and western blot and for a variety of in vitro applications, including inhibition and more.
Cystatin C (CST3, cystatin-3, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin, post-globulin G) is a small molecular weight peptide that is a potent inhibitor of many cysteine proteinases. Due to its small size, it is freely filtered by the glomeruli and metabolized after tubular reabsorption. Serum cystatin C levels are a more accurate test of kidney function than serum creatinine levels. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease.
It also appears to play a role in brain disorders related to amyloid deposition, such as Creutzfeldt-Jakob disease and Alzheimer’s disease. Molecular Innovations’ human cystatin C is recombinantly produced in insect cell culture and purified by conventional affinity and metal chelate chromatography. Contains a 10X histidine tag at the C-terminus for purification. It functions as a single band and is greater than 95% pure by SDS-PAGE. Inhibits the activity of the cysteine proteinase papain using the substrate Pyr-Phe-Leu-pNA.
Product name
C-terminal 10xHis-tagged recombinant
Full product name
N-terminal 10xHis-SUMO-tag and C-terminal Myc-tag
For research use only
For research use only. It should not be used in diagnostic procedures.
Host: E. coli
ISO Certification
Manufactured in an ISO 9001:2015 certified laboratory.
Other notes
Small volumes of N-terminal 10xHis-SUMO-tag and C-terminal Myc-tag Negative Control vials may occasionally become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.
Precautions
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Disclaimer
While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource shall not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without notice. It is the customer’s responsibility to report product performance issues to MyBioSource within 30 days of receiving the product.