yfp

Description: Rabbit polyclonal YFP antibody

Description: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Description: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Description: Topaz1, a yellow fluorescence protein (YFP), is one of the brightest and thermally stable green fluorescence proteins (GFP). The YFP (Topaz) Reporter Lentivirus contains replication-defective, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an EF1α promoter-driven YFP(Topaz) construct (a.a. sequence below), and confer both YFP (Topaz) expression and puromycin resistance to the target cells. YFP (Topaz) expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. Topaz has an excitation wavelength of 514 nm, an emission wavelength of 527 nm, and extinction coefficient of 94,500M-1cm-1._x000D_ _x000D_

Description: pre-made YFP expression adenovirus, provided in DMEM medium.

Description: Pre-made lentiviral particles expressing YFP with Neomycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Description: Pre-made lentiviral particles expressing yellow fluorescent protein (YFP), rovided in DMEM medium with 10% FBS, 60ug/ml polybrene.

Description: Pre-made lentiviral particles expressing YFP with Puromycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Description: Pre-made lentiviral particles expressing YFP under EF1a promoter with Blasticidin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Description: Pre-made lentiviral particles expressing YFP under EF1a promoter with Neomycin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Description: Pre-made lentiviral particles expressing YFP under EF1a promoter with Puromycin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Description: Concentrated Lentivirus express yellow fluorescent protein (YFP) under CMV promoter, containing Hygromycin selection