Phosphorylation of the translation initiation issue eIF2α is a fast and very important response to many varieties of stress, together with protein-misfolding stress in the endoplasmic reticulum (ER stress). It is believed to trigger a basic discount in protein synthesis whereas enabling translation of few transcripts. Such a discount of protein synthesis comes with the risk of depleting important proteins, a danger regarded as mitigated by its transient nature. Here, we discover that translation attenuation shouldn’t be uniform, with cytosolic and mitochondrial ribosomal subunits being prominently downregulated.

Translation attenuation of these targets persists after translation restoration. Surprisingly, this happens with no measurable lower in ribosomal proteins. Explaining this conundrum, translation attenuation preferentially targets long-lived proteins, a discovering not solely demonstrated by ribosomal proteins but additionally noticed at a worldwide stage. This exhibits that protein stability buffers the price of translational attenuation, establishing an evolutionary precept of mobile robustness. Somatic mutations in the RAS genes are frequent in human tumors, particularly in pancreatic, colorectal, and non-small-cell lung cancers.

Such mutations usually lower the capability of Ras to hydrolyze GTP, sustaining the protein in a constitutively energetic GTP-bound kind that drives uncontrolled cell proliferation. Efforts to develop medicine that focus on Ras oncoproteins have been unsuccessful. Recent rising knowledge recommend that Ras regulation is extra advanced than the scientific neighborhood has believed for many years. In this overview, we summarize advances in the “textbook” view of Ras activation. We additionally focus on a novel sort of Ras regulation that entails direct phosphorylation and dephosphorylation of Ras tyrosine residues. The discovery that pharmacological inhibition of the tyrosine phosphoprotein phosphatase SHP2 maintains mutant Ras in an inactive state means that SHP2 could possibly be a novel drug goal for the remedy of Ras-driven human cancers.

Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons

At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a kind of long-term despair (mGluR-LTD) that depends on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the growth of psychological problems akin to autism spectrum problems and subsequently advantage a greater understanding on a molecular stage. Here, to review mGluR-induced signaling pathways, we built-in quantitative phosphoproteomics with the analyses of newly synthesized proteins through bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling technique in cultured hippocampal neurons stimulated with DHPG, a particular agonist for group I mGluRs.

We recognized a number of kinases with necessary roles in DHPG-induced mGluR activation, which we confirmed utilizing small molecule kinase inhibitors. Furthermore, adjustments in the AMPA receptor endocytosis pathway in each protein synthesis and protein phosphorylation have been recognized, whereby Intersectin-1 was validated as a novel participant on this pathway. This examine revealed a number of new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and supplies a wealthy useful resource for additional analyses. The intracellular parasite Toxoplasma gondii resides inside a membrane-bound parasitophorous vacuole (PV) and secretes an array of proteins to determine this replicative area of interest.

It has been proven beforehand that Toxoplasma secretes kinases and that quite a few proteins are phosphorylated after secretion. Here, we assess the position of the phosphorylation of strand-forming protein 1 (SFP1) and the associated protein GRA29, two secreted proteins with unknown perform. We present that each proteins kind stranded constructions in the PV which are unbiased of the beforehand described intravacuolar community or actin. SFP1 and GRA29 can every kind these constructions independently of different Toxoplasma secreted proteins, though GRA29 seems to control SFP1 strands. We present that an unstructured area at the C termini of SFP1 and GRA29 is required for the formation of strands and that mimicking the phosphorylation of this area of SFP1 negatively regulates strand growth.

When tachyzoites convert to chronic-stage bradyzoites, each proteins present a dispersed localization all through the cyst matrix. Many secreted proteins are reported to dynamically redistribute as the cyst varieties, and secreted kinases are recognized to play a task in cyst formation. Using quantitative phosphoproteome and proteome analyses evaluating tachyzoite and early bradyzoite phases, we reveal widespread differential phosphorylation of secreted proteins. While we discovered no direct proof for phosphorylation taking part in a dominant position for SFP1/GRA29 redistribution in the cyst, these knowledge help a mannequin during which secreted kinases and phosphatases contribute to the regulation of secreted proteins throughout stage conversion.

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation

Greenbug (Schizaphis graminum) herbivory considerably impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum)

Switchgrass (Panicum virgatum L.) is a crucial crop for biofuel manufacturing nevertheless it additionally serves as host for greenbugs (Schizaphis graminum Rondani; GB). Although transcriptomic research have been achieved to deduce the molecular mechanisms of plant protection in opposition to GB, little is understood about the impact of GB infestation on the switchgrass protein expression and phosphorylation regulation.

Maltose Binding Protein Antibody

48666-100ul 100ul
EUR 333

Maltose Binding Protein Antibody

48666-50ul 50ul
EUR 239

Maltose Binding Protein Antibody

abx022501-02mg 0.2 mg
EUR 704
  • Shipped within 5-10 working days.

Maltose Binding Protein Antibody

abx023318-1mg 1 mg
EUR 1233
  • Shipped within 5-10 working days.

Maltose-Binding protein Antibody

20-abx137166
  • EUR 1024.00
  • EUR 495.00
  • EUR 676.00
  • 100 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Maltose-Binding protein Antibody

20-abx137495
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

E.Coli Maltose Binding Protein

20-abx262334
  • EUR 829.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-10 working days.

Rabbit Anti Maltose Binding Protein Polyclonal Antibody

DPBT-68161RM 0.1 ml
EUR 715

Anti-Maltose Binding Protein Rabbit Monoclonal Antibody

M30934 100ug/vial
EUR 397
Description: Rabbit Monoclonal Maltose Binding Protein Antibody. Validated in WB and tested in E. Coli.

Anti-Maltose Binding Protein Rabbit Monoclonal Antibody

M30934-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Maltose Binding Protein Antibody. Validated in WB and tested in Human, Mouse, Rat.

Maltose Binding Protein (MBP) Protein

20-abx655552
  • EUR 411.00
  • EUR 217.00
  • EUR 1052.00
  • EUR 467.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Recombinant E.Coli Maltose Binding Protein

7-05572 5µg Ask for price

Recombinant E.Coli Maltose Binding Protein

7-05573 200µg Ask for price

Recombinant E.Coli Maltose Binding Protein

7-05574 1mg Ask for price

Maltose Binding Protein Conjugated Antibody

C48666 100ul
EUR 397

Monoclonal Anti-Maltose binding protein (MBP)-AP conjugate

MBP11-AP 100 ul
EUR 347

Monoclonal Anti-Maltose binding protein (MBP)-HRP conjugate

MBP11-HRP 100 ul
EUR 347

Monoclonal Anti-Maltose binding protein (MBP) (fusion tag) ascites

MBP11-M 100 ul
EUR 482

Rabbit Anti-Maltose binding protein (MBP) (fusion tag) IgG

MBP12-A 100 ug
EUR 482

E. coli Maltose Binding Protein (MBP) Antibody

35738-05111 150 ug
EUR 261

Escherichia coli Maltose-binding periplasmic protein (malE)

1-CSB-EP360183ENV
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 48.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Escherichia coli Maltose-binding periplasmic protein(malE) expressed in E.coli

Maltose

HY-N2024 100mg
EUR 108

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

MBP Maltose Binding Protein (27-396) E.coli Recombinant Protein

PROTP0AEX9 Regular: 20ug
EUR 317
Description: Recombinant E.Coli MBP produced in E.Coli is a single, non-glycosylated polypeptide chain containing 371 amino acids (27-396 a.a) and having a molecular mass of 40.8kDa. ;MBP protein was purified by proprietary chromatographic techniques.

Recombinant other Maltose Binding Protein Protein, Untagged, E.coli-1mg

QP12656-1mg 1mg
EUR 617

Recombinant other Maltose Binding Protein Protein, Untagged, E.coli-200ug

QP12656-200ug 200ug
EUR 201

Recombinant other Maltose Binding Protein Protein, Untagged, E.coli-50ug

QP12656-50ug 50ug
EUR 155

Recombinant other Maltose Binding Protein Protein, His, E.coli-20ug

QP12656-HIS-EC-20ug 20ug
EUR 201

Recombinant other Maltose Binding Protein Protein, His, E.coli-5ug

QP12656-HIS-EC-5ug 5ug
EUR 155

Recombinant other Maltose Binding Protein Protein, Untagged, E.coli-1mg

QP12657-1mg 1mg
EUR 2757

Recombinant other Maltose Binding Protein Protein, Untagged, E.coli-20ug

QP12657-20ug 20ug
EUR 201

Recombinant other Maltose Binding Protein Protein, Untagged, E.coli-5ug

QP12657-5ug 5ug
EUR 155

D-Maltose

B7884-100 100 mg
EUR 108

D-Maltose

B7884-500 500 mg
EUR 166

Maltose monohydrate

HY-N2024A 100mg
EUR 108

Maltose Phosphorylase

M46630002 250U
EUR 115.25
  • Product category: Proteins/Enzymes/Glycosyltransferases

Maltose Phosphorylase

M46631002 1KU
EUR 226.18
  • Product category: Proteins/Enzymes/Glycosyltransferases

Maltose, monohydrate

MIB1184 100g
EUR 65.66
  • Product category: Biochemicals/Carbohydrates/Sugars

Maltose Binding Protein - Tag (MBP-Tag) Antibody (Clone 17D07)

6715-100
EUR 403

E. coli Maltose Binding Protein (MBP) Antibody (Biotin Conjugate)

35738-05121 150 ug
EUR 369

Maltose binding protein (MBP) (fusion tag) control for western

MBP11-C 100 ul
EUR 286

Maltose binding protein (MBP) (fusion tag) control for ELISA

MBP15-R-100 100 ug
EUR 347

Maltose binding protein (MBP) (fusion tag) control for ELISA

MBP15-R-1000 1 mg
EUR 773

MBP E.Coli, Maltose Binding Protein E.coli Recombinant Protein, His Tag

PROTP0AEX9-1 Regular: 20ug
EUR 317
Description: Recombinant E.Coli MBP produced in E.Coli is a single, non-glycosylated polypeptide chain containing 410 amino acids (27-392 a.a) and having a molecular mass of 44.9kDa. ;MBP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

E. coli Maltose Binding Protein (MBP) AssayLite Antibody (FITC Conjugate)

35738-05141 150 ug
EUR 428

E. coli Maltose Binding Protein (MBP) AssayLite Antibody (RPE Conjugate)

35738-05151 150 ug
EUR 428

E. coli Maltose Binding Protein (MBP) AssayLite Antibody (APC Conjugate)

35738-05161 150 ug
EUR 428

E. coli Maltose Binding Protein (MBP) AssayLite Antibody (PerCP Conjugate)

35738-05171 150 ug
EUR 471

Maltose Assay Kit

55R-1460 100 assays
EUR 723
Description: Assay Kit for detection of Maltose in the research laboratory

Maltose Assay Kit

55R-1470 100 assays
EUR 706
Description: Assay Kit for detection of Maltose in the research laboratory

D-(+)-Maltose monohydrate

abx082034-100g 100 g
EUR 189
  • Shipped within 5-10 working days.

D-(+)-Maltose monohydrate

abx082249-100g 100 g
EUR 189
  • Shipped within 5-10 working days.

D-(+)-Maltose, monohydrate

MB4950 100g
EUR 66.53
  • Product category: Biochemicals/Carbohydrates/Sugars

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

D-(+)-Maltose monohydrate, 95%

GC1102-1KG 1 kg
EUR 86

D-(+)-Maltose monohydrate, 95%

GC1102-500G 500 g
EUR 62

EnzyChrom Maltose Assay Kit

EMLT-100 100
EUR 351
Description: determination of maltose in various biological samples such as serum, urine, food and beverages, etc.

Maltose Dehydrogenase (r-MDH)

Y46755003 50KU
EUR 637.25
  • Product category: Proteins/Enzymes/Dehydrogenases

Maltose Dehydrogenase (r-MDH)

Y46756003 10KU
EUR 173.98
  • Product category: Proteins/Enzymes/Dehydrogenases

TruStrip WBV Maltose Binding protein quantitation & (MBP) Western Blot Validation Strips (10/pk)

WBMBP10-10 1 Pk
EUR 225

Maltose Colorimetric/Fluorometric Assay Kit

K628-100
EUR 512

Maltose Phosphorylase Activity Kit (Fluorometric)

K353-100
EUR 533

Maltose Colorimetric/Fluorometric Assay Kit

K2132-100 100 assays
EUR 502

D-(+)-Maltose monohydrate, USP/NF grade

GC5556-100G 100 g
EUR 44

D-(+)-Maltose monohydrate, USP/NF grade

GC5556-10KG 10 kg
EUR 381

D-(+)-Maltose monohydrate, USP/NF grade

GC5556-1KG 1 kg
EUR 86

D-(+)-Maltose monohydrate, USP/NF grade

GC5556-250G 250 g
EUR 54

D-(+)-Maltose monohydrate, USP/NF grade

GC5556-25KG 25 kg
EUR 732

D-(+)-Maltose monohydrate, USP/NF grade

GC5556-5KG 5 kg
EUR 245

anti-C4 binding protein

YF-PA10570 50 ug
EUR 363
Description: Mouse polyclonal to C4 binding protein

anti-C4 binding protein

YF-PA10571 100 ul
EUR 403
Description: Rabbit polyclonal to C4 binding protein

anti-C4 binding protein

YF-PA10572 100 ug
EUR 403
Description: Rabbit polyclonal to C4 binding protein

anti-Folate Binding Protein

YF-PA11838 50 ug
EUR 363
Description: Mouse polyclonal to Folate Binding Protein

anti-Folate Binding Protein

YF-PA11839 100 ug
EUR 403
Description: Rabbit polyclonal to Folate Binding Protein

anti-MAD2L1 binding protein

YF-PA16419 50 ug
EUR 363
Description: Mouse polyclonal to MAD2L1 binding protein

anti-MAD2L1 binding protein

YF-PA16420 50 ug
EUR 363
Description: Mouse polyclonal to MAD2L1 binding protein

anti-MAD2L1 binding protein

YF-PA16421 100 ul
EUR 403
Description: Rabbit polyclonal to MAD2L1 binding protein

anti-MAD2L1 binding protein

YF-PA16422 100 ug
EUR 403
Description: Rabbit polyclonal to MAD2L1 binding protein

anti-S100P binding protein

YF-PA20577 50 ug
EUR 363
Description: Mouse polyclonal to S100P binding protein

anti-C4 binding protein

YF-PA23331 50 ul
EUR 334
Description: Mouse polyclonal to C4 binding protein

anti-MAX binding protein

YF-PA24163 50 ul
EUR 334
Description: Mouse polyclonal to MAX binding protein

Maltose and Glucose Colorimetric/Fluorometric Assay Kit

K618-100
EUR 528

Mouse Anti-C4 binding protein A Polyclonal Antibody

CTA-450-100ug 100ug Ask for price
Description: Mouse anti-C4 binding protein A polyclonal antibody for WB.

Mouse Anti-C4 binding protein A Polyclonal Antibody

CTA-450-1mg 1mg Ask for price
Description: Mouse anti-C4 binding protein A polyclonal antibody for WB.

Mouse Anti immunobulin Binding Protein antibody ELISA kit

E03B0030-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Anti immunobulin Binding Protein antibody ELISA kit

E03B0030-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Anti immunobulin Binding Protein antibody ELISA kit

E03B0030-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Anti immunobulin Binding Protein antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-CCAAT/enhancer binding protein zeta (mouse) antibody

STJ72907 100 µg
EUR 359

Anti-eEF1A2 binding protein Antibody

A14038 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for eEF1A2 binding protein Antibody (IGFN1) detection. Tested with WB, IHC in Human, Mouse, Rat, Pig.

anti-Retinol binding protein (20F9)

LF-MA0162 100 ul
EUR 334
Description: Mouse monoclonal to Retionol binding protein

anti-eEF1A2 binding protein (5B12)

LF-MA10086 100 ug
EUR 363
Description: Mouse monoclonal to eEF1A2 binding protein

Anti-Csk binding protein antibody

STJ16100205 100 µg
EUR 524

Anti-Csk-binding protein antibody

STJ16101301 100 µg
EUR 524

Anti-Csk-binding protein antibody

STJ16101434 100 µg
EUR 524

Anti-eEF1A2 binding protein antibody

STJ97490 200 µl
EUR 197
Description: Rabbit polyclonal to eEF1A2 binding protein.

The world response of the switchgrass cultivar Summer proteome and phosphoproteome was monitored by label-free proteomics shotgun in GB-infested and uninfested management vegetation at 10 days submit infestation. Peptides matching a complete of 3,594 proteins have been recognized and 429 have been differentially expressed proteins in GB-infested vegetation relative to uninfested management vegetation. Among these, 291 and 138 have been up and downregulated by GB infestation, respectively. Phosphoproteome evaluation recognized 310 differentially phosphorylated proteins (DP) from 350 phosphopeptides with a complete of 399 phosphorylated websites.

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation
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