Nucleotide-binding area and leucine-rich repeat-containing (NLR) proteins perform as sensors that understand pathogen molecules and activate immunity. In vegetation, the buildup and activation of NLRs is regulated by SUPPRESSOR OF G2 ALLELE OF skp1 (SGT1). In this work, we discovered that an effector protein named RipAC, secreted by the plant pathogen Ralstonia solanacearum, associates with SGT1 to suppress NLR-mediated SGT1-dependent immune responses, together with these triggered by one other R. solanacearum effector, RipE1. RipAC doesn’t have an effect on the buildup of SGT1 or NLRs, or their interplay. However, RipAC inhibits the interplay between SGT1 and MAP kinases, and the phosphorylation of a MAPK goal motif within the C-terminal area of SGT1.

Such phosphorylation is enhanced upon activation of immune signaling and contributes to the activation of immune responses mediated by the NLR RPS2. Additionally, SGT1 phosphorylation contributes to resistance in opposition to R. solanacearum. Our outcomes shed gentle onto the mechanism of activation of NLR-mediated immunity, and counsel a optimistic suggestions loop between MAPK activation and SGT1-dependent NLR activation. Clathrin-mediated and caveolar endocytic pathways signify the key routes by way of which G protein-coupled receptors (GPCRs) could possibly be internalized.

GPCR kinase 2 (GRK2) and β-arrestins are consultant proteins that mediate the GPCR endocytosis. However, the molecular mechanisms by way of which GRK2 and β-arrestin mediate clathrin-mediated and caveolar endocytosis stay unclear. In this research, we decided the mobile parts and processes that mediate the selective interplay between clathrin/caveolin1 and GRK2/β-arrestins. For this we utilized the next: (i) mutant dopamine D2 receptor and β2 adrenoceptor during which the potential GRK2 phosphorylation websites have been altered and (ii) cells during which clathrin, caveolin1, β-arrestins, or Mdm2 expression have been knocked down.

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus

The current research aimed to look at the consequences of FcγRIIB on systemic lupus erythematosus (SLE) and to examine the underlying mechanisms. For this function, lentiviral vector carrying the membrane‑certain sort FcγRIIB gene (mFcγRIIB lentivirus) and soluble FcγRIIB (sFcγRIIB) protein have been used to deal with B cells from sufferers with SLE. The B cells have been handled with calf thymus DNA (ctDNA) and anti‑calf thymus DNA‑immune complexes (anti‑ctDNA‑IC). mFcγRIIB lentivirus and sFcγRIIB protein have been additionally injected into MRL/lpr SLE mice.

The outcomes revealed that anti‑ctDNA‑IC therapy considerably downregulated the IgG antibody secretion of B cells handled with mFcγRIIB lentivirus. mFcγRIIB and sFcγRIIB decreased the phosphorylation stage of Bruton’s tyrosine kinase (BTK) in B cells, and elevated the phosphorylation stage of Lyn proto‑oncogene (Lyn), docking protein 1 (DOK1) and inositol polyphosphate‑5‑phosphatase D (SHIP). mFcγRIIB promoted the apoptosis of B cells. Following the therapy of MRL/lpr SLE mice with mFcγRIIB lentivirus, the degrees of urinary protein, serum anti‑nuclear and anti‑dsDNA antibodies have been decreased, whereas the degrees of mFcγRIIB in B cells have been elevated. mFcγRIIB ameliorated the pathologies of the kidneys, liver and lymph node tissues of the MRL/lpr SLE mice.

Following therapy of the MRL/lpr SLE mice with sFcγRIIB, the degrees of urinary protein, serum anti‑dsDNA antibody and BTK and SHIP phosphorylation ranges in B cells have been decreased, whereas the serum sFcγRIIB and sFcγRIIB‑IgG ranges have been elevated. On the entire, the findings of the current research show that recombinant FcγRIIB inhibits the secretion of IgG antibody by B cells from sufferers with SLE, ameliorates the signs of SLE in mice, and alters the phosphorylation ranges of downstream proteins of the FcγRIIB signaling pathway in B cells. These outcomes counsel that FcγRIIB might play preventive and therapeutic roles in SLE by inhibiting B cell activation by way of the FcγRIIB signaling pathway, which offers a novel principle and technique for the prevention and therapy of SLE.

A bacterial effector protein prevents MAPK-mediated phosphorylation of SGT1 to suppress plant immunity

Explicit illustration of protein exercise states considerably improves causal discovery of protein phosphorylation networks

Protein phosphorylation networks play an essential position in cell signaling. In these networks, phosphorylation of a protein kinase normally leads to its activation, which in flip will phosphorylate its downstream goal proteins. A phosphorylation community is actually a causal community, which might be discovered by causal inference algorithms. Prior efforts have utilized such algorithms to knowledge measuring protein phosphorylation ranges, assuming that the phosphorylation ranges signify protein exercise states. However, the phosphorylation standing of a kinase doesn’t at all times replicate its exercise state, as a result of interventions equivalent to inhibitors or mutations can instantly have an effect on its exercise state with out altering its phosphorylation standing.
Thus, when mobile techniques are subjected to intensive perturbations, the statistical relationships between phosphorylation states of proteins could also be disrupted, making it troublesome to reconstruct the true protein phosphorylation community. Here, we describe a novel framework to deal with this problem. We have developed a causal discovery framework that explicitly represents the exercise state of every protein kinase as an unmeasured variable and developed a novel algorithm referred to as “InferA” to infer the protein exercise states, which permits us to incorporate the protein phosphorylation stage, pharmacological interventions and prior information.
We utilized our framework to simulated datasets and to a real-world dataset. Our outcomes confirmed that clathrin-mediated endocytosis happens extra quickly than caveolar endocytosis. Clathrin-mediated endocytosis and the interplay between clathrin and GRK2/β-arrestin2 occurred in a GRK2-mediated receptor phosphorylation-dependent method.  In distinction, caveolar endocytosis and the interplay between caveolin1 and GRK2/β-arrestin2 have been impartial of receptor phosphorylation standing.
Recombinant Protein G
7-05936 10mg Ask for price
Recombinant Protein G
7-05937 100mg Ask for price
Recombinant Protein A
DAG390 1g
EUR 1940
PTPN1 Protein (Recombinant)
20-abx073070
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.
PPM1A Protein (Recombinant)
20-abx073071
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.
PTP4A3 Protein (Recombinant)
20-abx073078
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.
SKP1 Protein (Recombinant)
20-abx073140
  • EUR 2861.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Ketohexokinase Protein (Recombinant)
20-abx073142
  • EUR 2861.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
NGAL Protein (Recombinant)
20-abx073150
  • EUR 328.00
  • EUR 3084.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
DDAH1 Protein (Recombinant)
20-abx073166
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PTP4A1 Protein (Recombinant)
20-abx073184
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP3R1 Protein (Recombinant)
20-abx073192
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPM1F Protein (Recombinant)
20-abx073211
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPME1 Protein (Recombinant)
20-abx073232
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP4C Protein (Recombinant)
20-abx073279
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
ACAD8 Protein (Recombinant)
20-abx073280
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
ACAT2 Protein (Recombinant)
20-abx073281
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
RNASE3 Protein (Recombinant)
20-abx073373
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
MAPKAPK3 Protein (Recombinant)
20-abx073468
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PRKAB2 Protein (Recombinant)
20-abx073469
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
ATF3 Protein (Recombinant)
20-abx073484
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
DYRK1A Protein (Recombinant)
20-abx073488
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PTPN6 Protein (Recombinant)
20-abx073509
  • EUR 3530.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
NGAL Protein (Recombinant)
20-abx073513
  • EUR 3766.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
NGAL Protein (Recombinant)
20-abx073525
  • EUR 328.00
  • EUR 4281.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
PTPMT1 Protein (Recombinant)
20-abx073545
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP1R14A Protein (Recombinant)
20-abx073553
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP1R2 Protein (Recombinant)
20-abx073582
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PRKA1RA Protein (Recombinant)
20-abx073603
  • EUR 4615.00
  • EUR 230.00
  • EUR 328.00
  • 100 ug
  • 1 µg
  • 3 µg
  • Shipped within 5-10 working days.
PPARG Protein (Recombinant)
20-abx073662
  • EUR 328.00
  • EUR 5924.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
PPP3R2 Protein (Recombinant)
20-abx073701
  • EUR 6397.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
ACADL Protein (Recombinant)
20-abx073728
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
ACADVL Protein (Recombinant)
20-abx073752
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
ACADS Protein (Recombinant)
20-abx073767
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
ACADM Protein (Recombinant)
20-abx073777
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
ACADSB Protein (Recombinant)
20-abx073808
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
PPP1R8 Protein (Recombinant)
20-abx073809
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
ATF4 Protein (Recombinant)
20-abx073858
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
CDK2 Protein (Recombinant)
20-abx073859
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
FGFR1 Protein (Recombinant)
20-abx073878
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
Ribokinase Protein (Recombinant)
20-abx073896
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
ADK Protein (Recombinant)
20-abx073897
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
PTP4A2 Protein (Recombinant)
20-abx073900
  • EUR 6397.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
PTPN7 Protein (Recombinant)
20-abx073941
  • EUR 230.00
  • EUR 1483.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP3CA Protein (Recombinant)
20-abx073950
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP1CA Protein (Recombinant)
20-abx073956
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.
ABHD14B Protein (Recombinant)
20-abx073961
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.
PPP1CC Protein (Recombinant)
20-abx073968
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.
PTPRC Protein (Recombinant)
20-abx073990
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.
SCCA recombinant protein
E62C01501 20ug
EUR 382
SCCA1 recombinant protein
E62C01502 20ug
EUR 382
SCCA2 recombinant protein
E62C01503 20ug
EUR 382
P53 recombinant protein
E62C02301 20ug
EUR 382
DKK1 recombinant protein
E62C02401 20ug
EUR 382
Staphylokinase Recombinant Protein
PROTP68802 Regular: 100ug
EUR 317
Description: Staphylokinase Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 136 amino acids and having a molecular weight of 16kDa.;The Staphylokinase is purified by proprietary chromatographic techniques.
TIAL1Human Recombinant Protein
PROTQ01085 Regular: 20ug
EUR 317
Description: TIAL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-375 a.a) and having a molecular mass of 44.0kDa.TIAL1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LIN7BHuman Recombinant Protein
PROTQ9HAP6 Regular: 10ug
EUR 317
Description: LIN7B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-207 a.a.) and having a molecular mass of 25.3kDa.;LIN7B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
KIAA0513Human Recombinant Protein
PROTO60268 Regular: 10ug
EUR 317
Description: KIAA0513 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (1-401 a.a) and having a molecular mass of 47.9kDa.;KIAA0513 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Batroxobin Recombinant Protein
PROTP04971 Regular: 10ug
EUR 317
Description: The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosilated polypeptide chain containing 231 amino acids and having an Mw of approximately 28-33 kDa.
Lysostaphin Recombinant Protein
PROTP10547 Regular: 5mg
EUR 366
Description: Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.;The Mw of lysostaphin is 26,921 (Recsei et al, PNAS 1987).
Streptavidin Recombinant Protein
PROTP22629-2 Regular: 20mg
EUR 317
Description: Streptavidin Streptomyces Avidinii Recombinant produced in E.Coli. ;The molecular weight per tetramer is approximately 52kDa.
Recombinant Echovirus Protein
VAng-Lsx0595-inquire inquire Ask for price
Description: Echovirus, recombinant protein from E. coli.
Recombinant RSV Protein
VAng-Lsx0448-inquire inquire Ask for price
Description: RSV, recombinant protein from Hep-2 Cells.
Recombinant Rotavirus Protein
VAng-Lsx0471-inquire inquire Ask for price
Description: RotaVirus, recombinant protein from MA 104 cells.
Recombinant SIV Protein
VAng-Lsx0493-inquire inquire Ask for price
Description: Recombinant SIV p55- Strains: SIV mac 23g and SIV smH4 is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells.
Recombinant Coxsackievirus Protein
VAng-Lsx0074-inquire inquire Ask for price
Description: Coxsackievirus antigen, recombinant protein from E. coli.
Recombinant EBV Protein
VAng-Lsx0108-inquire inquire Ask for price
Description: EBV, recombinant protein from human cells.
Recombinant EBOV Protein
VAng-Lsx0136-1mg 1 mg
EUR 1138
Description: EBOV, recombinant protein from E. coli.
Recombinant Hantavirus Protein
VAng-Lsx0152-100gEcoli 100 µg (E. coli)
EUR 670
Description: Hantavirus, recombinant protein from E. coli.
Recombinant Hantavirus Protein
VAng-Lsx0152-1mgEcoli 1 mg (E. coli)
EUR 3402
Description: Hantavirus, recombinant protein from E. coli.
Recombinant Hantavirus Protein
VAng-Lsx0152-500gEcoli 500 µg (E. coli)
EUR 1787
Description: Hantavirus, recombinant protein from E. coli.
Recombinant HBV Protein
VAng-Lsx0175-1mg 1 mg
EUR 3470
Description: HBV, recombinant protein from E. coli.
Recombinant HDV Protein
VAng-Lsx0220-inquire inquire Ask for price
Description: HDV, recombinant protein from E. coli.
TAGLN Recombinant Protein (Rat) (Recombinant- Tag)
RP232205 100 ug Ask for price
TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)
RP232208 100 ug Ask for price
TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)
RP232211 100 ug Ask for price
TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)
RP232214 100 ug Ask for price
TAGAP Recombinant Protein (Human) (Recombinant- Tag)
RP043930 100 ug Ask for price
CTAGE3P Recombinant Protein (Human) (Recombinant Tag)
RP053400 100 ug Ask for price
CTAGE4 Recombinant Protein (Human) (Recombinant- Tag)
RP053403 100 ug Ask for price
CTAGE8 Recombinant Protein (Human) (Recombinant- Tag)
RP053409 100 ug Ask for price
CTAGE9 Recombinant Protein (Human) (Recombinant- Tag)
RP053412 100 ug Ask for price
STAG3L1 Recombinant Protein (Human) (Recombinant Tag)
RP030283 100 ug Ask for price
STAG3L2 Recombinant Protein (Human) (Recombinant Tag)
RP030286 100 ug Ask for price
STAG3L3 Recombinant Protein (Human) (Recombinant Tag)
RP030289 100 ug Ask for price
STAG3L4 Recombinant Protein (Human) (Recombinant Tag)
RP030292 100 ug Ask for price
TAGAP Recombinant Protein (Human) (Recombinant- Tag)
RP030880 100 ug Ask for price
TAGLN Recombinant Protein (Human) (Recombinant- Tag)
RP030883 100 ug Ask for price
TAGLN Recombinant Protein (Human) (Recombinant- Tag)
RP030886 100 ug Ask for price
TAGLN2 Recombinant Protein (Human) (Recombinant Tag)
RP030889 100 ug Ask for price
TAGLN2 Recombinant Protein (Human) (Recombinant Tag)
RP030892 100 ug Ask for price
TAGLN3 Recombinant Protein (Human) (Recombinant Tag)
RP030895 100 ug Ask for price
CTAG1A Recombinant Protein (Human) (Recombinant- Tag)
RP038332 100 ug Ask for price
CTAG1B Recombinant Protein (Human) (Recombinant- Tag)
RP038335 100 ug Ask for price
CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)
RP038338 100 ug Ask for price
CTAGE1 Recombinant Protein (Human) (Recombinant- Tag)
RP008266 100 ug Ask for price
CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)
RP008269 100 ug Ask for price
The simulation experiments demonstrated that express illustration of exercise states of protein kinases permits one to successfully signify the influence of interventions and thus enabled our framework to precisely recuperate the ground-truth causal community. Results from the real-world dataset confirmed that the express illustration of protein exercise states allowed an efficient and data-driven integration of the prior information by InferA, which additional leads to the restoration of a phosphorylation community that’s extra according to experiment outcomes.
A bacterial effector protein prevents MAPK-mediated phosphorylation of SGT1 to suppress plant immunity
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